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Eagle Biosciences donor serum vitamin d levels
Donor serum IgG specific to (A) cytomegalovirus (CMV) antigens and (B) the inducing antigen, recombinant human myelin oligodendrocyte glycoprotein (rhMOG 1-120 ). For A and B, group data are shown as mean with SEM (n = 3 – 4 donors/group) and were curve fit with a one-site total binding equation. Statistical differences in titre curves were assessed by ordinary two-way ANOVA. The lower limit of detection (LOD) is represented by a dashed line. (C) Donor serum 25-OH <t>vitamin</t> <t>D</t> levels. Data are shown as mean with SEM (n = 3 – 4 donors/group) and were analyzed by Brown-Forsythe and Welch ANOVA with Dunnett’s T3 multiple comparisons test. *p<0.05.
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Donor serum IgG specific to (A) cytomegalovirus (CMV) antigens and (B) the inducing antigen, recombinant human myelin oligodendrocyte glycoprotein (rhMOG 1-120 ). For A and B, group data are shown as mean with SEM (n = 3 – 4 donors/group) and were curve fit with a one-site total binding equation. Statistical differences in titre curves were assessed by ordinary two-way ANOVA. The lower limit of detection (LOD) is represented by a dashed line. (C) Donor serum 25-OH vitamin D levels. Data are shown as mean with SEM (n = 3 – 4 donors/group) and were analyzed by Brown-Forsythe and Welch ANOVA with Dunnett’s T3 multiple comparisons test. *p<0.05.

Journal: bioRxiv

Article Title: Epstein-Barr virus infection promotes Th1-mediated disease in a humanized immune mouse model of multiple sclerosis

doi: 10.1101/2022.02.23.481716

Figure Lengend Snippet: Donor serum IgG specific to (A) cytomegalovirus (CMV) antigens and (B) the inducing antigen, recombinant human myelin oligodendrocyte glycoprotein (rhMOG 1-120 ). For A and B, group data are shown as mean with SEM (n = 3 – 4 donors/group) and were curve fit with a one-site total binding equation. Statistical differences in titre curves were assessed by ordinary two-way ANOVA. The lower limit of detection (LOD) is represented by a dashed line. (C) Donor serum 25-OH vitamin D levels. Data are shown as mean with SEM (n = 3 – 4 donors/group) and were analyzed by Brown-Forsythe and Welch ANOVA with Dunnett’s T3 multiple comparisons test. *p<0.05.

Article Snippet: Donor serum vitamin D levels were quantified using the 25-OH Vitamin D ELISA assay test kit, according to the manufacturer’s instructions (Eagle Biosciences, #VID31-K01).

Techniques: Recombinant, Binding Assay